The development of therapeutic monoclonal antibodies against rabies virus from immunized phage-displayed libraries /
Apidsada Chorpunkul.
The development of therapeutic monoclonal antibodies against rabies virus from immunized phage-displayed libraries / Apidsada Chorpunkul. - Nakhonpathom : Mahidol University, 2023. - 276 p. : ill., tables, chart ; 30 cm.
Chapter 1 Introduction and objectives --
Chapter 2 Literature reviews --
Chapter 3 Materials and methods --
3.1 Sample collection and Preparation --
3.2 Plasma RFFIT assay and PBMC isolation --
3.3 Total RNA extraction and cDNA synthesis --
3.4 Variable heavy (VH) and light chain (VL, kappa and lambda) fragment amplification --
3.5 Generation of single chain variable fragments (scFv) --
3.6 Construction of scFv libraries --
3.7 The scFv phage display library construction --
3.8 Biopanning (Affinity selection) --
3.9 Specific binding ELISA of individual clones --
3.10 DNA sequencing and CDR determination --
3.11 Soluble scFv expression --
3.12 Purification of soluble scFv antibodies --
3.13 Modified Rapid Fluorescent Foci Inhibition Test (RFFIT): neutralization assay --
3.14 Sensitivity test and binding ability of neutralizing scFv clones by dot-blot ELISA --
3.15 Bioinformatics --
Chapter 4 Results --
4.1 Human therapeutic monoclonal antibodies against RABV --
4.2 Caine therapeutic monoclonal antibodies against RABV --
Chapter 5 Discussion --
Chapter 6 Conclusion.
Rabies
Rabies virus
QR 189.5.R3 / A64D 2023
The development of therapeutic monoclonal antibodies against rabies virus from immunized phage-displayed libraries / Apidsada Chorpunkul. - Nakhonpathom : Mahidol University, 2023. - 276 p. : ill., tables, chart ; 30 cm.
Chapter 1 Introduction and objectives --
Chapter 2 Literature reviews --
Chapter 3 Materials and methods --
3.1 Sample collection and Preparation --
3.2 Plasma RFFIT assay and PBMC isolation --
3.3 Total RNA extraction and cDNA synthesis --
3.4 Variable heavy (VH) and light chain (VL, kappa and lambda) fragment amplification --
3.5 Generation of single chain variable fragments (scFv) --
3.6 Construction of scFv libraries --
3.7 The scFv phage display library construction --
3.8 Biopanning (Affinity selection) --
3.9 Specific binding ELISA of individual clones --
3.10 DNA sequencing and CDR determination --
3.11 Soluble scFv expression --
3.12 Purification of soluble scFv antibodies --
3.13 Modified Rapid Fluorescent Foci Inhibition Test (RFFIT): neutralization assay --
3.14 Sensitivity test and binding ability of neutralizing scFv clones by dot-blot ELISA --
3.15 Bioinformatics --
Chapter 4 Results --
4.1 Human therapeutic monoclonal antibodies against RABV --
4.2 Caine therapeutic monoclonal antibodies against RABV --
Chapter 5 Discussion --
Chapter 6 Conclusion.
Rabies
Rabies virus
QR 189.5.R3 / A64D 2023
