000			01915nam a2200241 4500
003			NALT
005			20251224113502.0
008			251105b th ||||| |||| 00| 0 eng d
040			_aNALT
050			_aQR 189.5.R3 _bA64D 2023
100	0		_aApidsada Chorpunkul. _966885
245	1	0	_aThe development of therapeutic monoclonal antibodies against rabies virus from immunized phage-displayed libraries / _cApidsada Chorpunkul.
260			_aNakhonpathom : _bMahidol University, _c2023.
300			_a276 p. : _bill., tables, chart ; _c30 cm.
505	2		_aChapter 1 Introduction and objectives -- Chapter 2 Literature reviews -- Chapter 3 Materials and methods -- 3.1 Sample collection and Preparation -- 3.2 Plasma RFFIT assay and PBMC isolation -- 3.3 Total RNA extraction and cDNA synthesis -- 3.4 Variable heavy (VH) and light chain (VL, kappa and lambda) fragment amplification -- 3.5 Generation of single chain variable fragments (scFv) -- 3.6 Construction of scFv libraries -- 3.7 The scFv phage display library construction -- 3.8 Biopanning (Affinity selection) -- 3.9 Specific binding ELISA of individual clones -- 3.10 DNA sequencing and CDR determination -- 3.11 Soluble scFv expression -- 3.12 Purification of soluble scFv antibodies -- 3.13 Modified Rapid Fluorescent Foci Inhibition Test (RFFIT): neutralization assay -- 3.14 Sensitivity test and binding ability of neutralizing scFv clones by dot-blot ELISA -- 3.15 Bioinformatics -- Chapter 4 Results -- 4.1 Human therapeutic monoclonal antibodies against RABV -- 4.2 Caine therapeutic monoclonal antibodies against RABV -- Chapter 5 Discussion -- Chapter 6 Conclusion.
650		0	_aRabies _966905
650		0	_aRabies virus _966904
942			_2lcc _cRSH
996			_ajidapa.t _bACQSTF _c2025-11-05
996			_avaree.k _bLIBSTF _c2025-11-06
996			_ajamriang.p _bCATSTF _c2025-11-06
999			_c107428 _d107428
998			_j1020 _k นางจำเรียง ระวังสำโรง